Ay is a minor antagonist in Mr. Peabody & Sherman. He is King Tut's vizier, and he is voiced by Steve Valentine.
He first appeared telling Penny what happens in the marriage, as a tradition. But when Penny regretted for being part of the marriage due to the painful price, King Tut imprisoned Sherman and Mr. Peabody while the guards took Penny to the wedding. When the wedding began, he smiled evilly as he was planning to kill Penny with a sword. When Mr. Peabody and Sherman managed to escape, Ay believed them when they made a sound of Anubis until the mouth of the Anubis statue fell down and Ay saw who was behind it. When the trio ran away, he and the other Egyptians went after them but failed to catch them. Ay is later seen at the end, dressed like the Statue of Liberty while King Tut and the others play with the artifacts from New York City.
- Historically, he followed Tut as Pharaoh of Egypt.
- He was the brother of Amenhotep III who was the grandfather of Tut making Ay the great uncle of Tut.
- His great granddaughter, Nefertari married Rameses II and had 9 children with him (one of which was Amun-her-khepeshef).
- He was the last Pharaoh of the Amarna Period. His successor, Horemheb removed many monuments dedicated to the Pharaohs of the Amarna Period.