In the film, Gladys tries to kill RJ and his friends as she has a hatred for wild animals. At the climax, she and Dwayne are knocked into her yard by Vincent and the trio get stung by the Depelter Turbo.
Gladys has a personal hatred against wild animals, especially RJ and the gang. This is normal because they were stealing and invading her her stuff.
- She is similar to Cruella De Vil from One Hundred and One Dalmatians: Both are animal haters, and then later get arrested and they are both the main antagonists in their movies, Cruella is the main antagonist of A Hundred and One Dalmatians, and Gladys is the main antagonist of Over the Hedge.
|Over the Hedge|
|Over the Hedge • Hammy's Boomerang Adventure|
|RJ • Verne • Hammy • Stella • Ozzie • Heather • Vincent • Lou • Penny • Bucky, Spike and Quillo • Tiger • Nugent • Gladys Sharp • The Verminator|
|Over the Hedge • Hammy Goes Nuts|