In Puss in Boots, Jack and Jill are in the possession of the magic beans from the Jack and the Beanstalk story. Eventually, Puss, Kitty Softpaws, and Humpty Dumpty track them down in their piglet-filled cart and, after battling them, manage to steal to get into the giant's keep.
However, it is revealed later that Jack and Jill were in cahoots with Humpty and Kitty to take the Golden Goose and trick Puss into being imprisoned in his home town. Jack and Jill were seemingly just Humpty's minions. Later on, the Great Terror arrives to retrieve the Golden Goose, its baby. It is revealed that Jack and Jill were planning to betray Humpty all along, wanting more than just golden eggs but the actual goose. They try to steal the Goose when Humpty had a change of heart and tried to help Puss lure the Great Terror away from San Ricardo, holding their gun to Humpty's head, only for Kitty Softpaws to fight them off and then be stomped on by the Terror.
During the credits, Jack and Jill are seen in full body casts.
They are mean, short-tempered, greedy, conniving, nasty, and overall evil in nature. Jack is tired of thieving and wanted children. Jill is rude and inconsiderate when someone wants to stay in the hotel.
Jack has ginger hair and a short ginger beard. He wears a hat, a red shirt, and leather shoes. Jill has short brown hair and wears a long, filthy-looking blue dress.
- Jack & Jill are constantly thought to be the secondary antagonists of the film and as Humpty Dumpty's former henchmen. This is not true as Jack & Jill had bigger plans and were never Humpty's henchmen, but rather only pretended to be.
- Jack & Jill make an appearance in the non-canon Shrek 2 game.