Petunia Rhubarb (introduced 2005) is a red-headed rhubarb, but looks little like her namesake. Petunia is often seen with Larry as his love interest and first appeared in Duke and the Great Pie War in playing the part of Princess Petunia, the scorned "Ruth" figure waiting to be redeemed by Larry's "Boaz". Petunia has also sang alongside Larry in the Silly Song, "Where Have All The Staplers Gone?" on the video Pistachio - The Little Boy That Woodn't.
Petunia Rhubarb is voiced by Cydney Trent from her debut to 2014, She is voiced by Tress MacNeille since 2014.